Master essential animal cell culture techniques crucial for modern biological research and industrial applications. This comprehensive guide delves into fundamental principles, advanced mammalian cell culture methods, media preparation, and critical quality control procedures for successful cell line maintenance and experimentation. Explore practical lab protocols and discover real-world applications directly relevant to advancements in Indian biotechnology and life sciences. An indispensable resource for undergraduate and postgraduate students in biotechnology, life sciences, and related disciplines, as well as researchers and professionals seeking to enhance their cell culture expertise in India.